Personal Trainer
The best advice is to assess your nutritional requirements by seeking professional guidance. NO amount of sit ups will give you a flat stomach. There are several factors to success, read this article for more information on creating a flat stomach: http://www.nataliecartertalksfitness.com/2011/10/create-flat-stomach-part-1.html
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As Natalie mentioned, toning of the midsection is mostly a product of weight loss but increasing abdominal muscle size will provide some definition.
Sit ups do utilize the abdominals, though the hip flexors are highly active. Try swiss ball sit ups to increase the abdominal component
It is best to develop the entire mid section (abs, obliques and lower back) in your exercise program. If you have no current injuries id recommend a swiss ball crunch for the upper abs, a reverse crunch or toe point for the lower, some side bridges or russian twists for the obliques and hip raises for the lower back. You can see all these exercises at this exercise library, along with progressions and regressions of each
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